Culture cell morphology and morphology analysis

In vitro cultured cells can be divided into two types: adherent growth and suspension growth according to whether they can be attached to a support in a culture vessel. Adherent cells can adhere to the surface of the branch and grow during culture. For example, amniotic fluid cells are adherent cells, which usually show the growth of fibroblasts and epithelial cells. Suspended cells grow in suspension in culture.

1. Fibroblasts

Cells in culture can be called fibroblasts when their morphology is similar to fibroblasts. This type of cell is named after its morphology is similar to that of fibroblasts in the body. The cell grows in a fusiform or irregular triangle shape on the surface of the support. There is an oval-shaped nucleus in the center of the cell. Protrusions of different lengths, in addition to true fibroblasts, all tissue cells originating from the mesoderm interstitial often grow in this type of morphology.

2. Epithelial cells

This type of cell grows on a culture vessel support with a flat, irregular polygonal shape. The center of the cell has a round nucleus, and the cells are closely connected with a single layer of membrane-like growth. Tissue cells originating from endoderm and ectoderm cells, such as skin, epidermal derivatives, and digestive tract epithelium, grow in an epithelial form.

3. Roaming cells

The cells of this type grow scattered on the support, and are generally not connected into pieces. The cytoplasm often protrudes pseudopods or protuberances, and exhibits active roaming or deforming movements. It is fast and irregular. This type of cell is not very stable, and sometimes it is difficult to distinguish it from other types of cells. Under certain conditions, due to the increase in cell density, the combined tablets can be shaped like a polygon or a fine fiber-forming package. Common in the early stage of amniotic fluid cell culture.

Culture cell morphology analysis

The cultured cells have different shapes depending on the shape of the attached support. The most common is the attachment of cells to a planar support. Under normal light microscope, the cells that are living are homogeneous and transparent, and the structure is not obvious. When there are 1-2 nucleoli in the growth phase of the cell, when the cell function is poor, the cell contour will increase and the contrast will increase. If particles, drips, and vesicles appear in the cytoplasm from time to time, it indicates poor cell metabolism.

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